ThromboLUX® uses the principle of dynamic light scattering (DLS) to determine the size and distribution of particles that are in a platelet sample.
To operate ThromboLUX, the user first prepares a sample in a single use capillary by following the instructions for use supplied with the Test Kit. The capillary is sealed and inserted into the Console. ThromboLUX displays step-by-step instructions on the LCD screen to guide the user through the sample identification process and start the test.
During the 20 minute test ThromboLUX performs measurements at 37°C, then 20°C, then again at 37°C. ThromboLUX uses a proprietary algorithm to calculate and analyze the size distribution at each test temperature. The following parameters are displayed on the screen:
- Photon rate which corresponds to the overall particle concentration in the suspension
- Mean particle size for each population
- Gated microparticle size and intensity
- Gated platelet size and intensity
- MP factor which can be used to calculate an approximate microparticle concentration
- The ThromboLUX Score which ranges from 0 to 40
Platelet concentrates with a high score are homogeneous and contain mostly discoid, functional platelets, whereas concentrates with a low score have a heterogeneous composition usually with a high microparticle content.