Applications

Platelet-Rich Plasma (PRP) Testing

ThromboLUX for PRP therapies

Platelets are used in a wide variety of autologous treatments, however the desired clinical outcomes are not always achieved. There are limited options for evaluating platelet preparations, and due to cost, complexity and accessibility, these techniques cannot be used routinely for point-of-care testing.

ThromboLUX® was developed as a cost effective tool to test platelets quickly at the point-of-care. ThromboLUX can be used to evaluate platelet products prior to use by providing physicians with information about the composition and relative concentration of the products.

ThromboLUX supplies previously unavailable information about platelet products, and has the potential to offer insight when using PRP injections as a therapy.

What does ThromboLUX do?

Determines composition

ThromboLUX characterizes the platelet product by determining the average size of cells and particles in the suspension.

Figure 5.5  a) Homogeneous platelets

Figure 5.5  a) Homogeneous platelets

Figure 5.5  b) Microparticles and microaggregates 

Figure 5.5  b) Microparticles and microaggregates 

Figure 5.5 c) Heterogeneous—possible contamination

Figure 5.5 c) Heterogeneous—possible contamination

Determines relative concentration

ThromboLUX uses dynamic light scattering to measure platelet samples. Particle concentrations are proportional to scattering intensity, and can be approximated using the photon count.

Figure 5.7 As particle concentration increases, light scattering (measured as photon rate) increases

Figure 5.7 As particle concentration increases, light scattering (measured as photon rate) increases

Figure 5.8 Platelet count is proportional to photon rate

Figure 5.8 Platelet count is proportional to photon rate

Why use ThromboLUX for PRP analysis?

Evidence from testing normal volunteer blood donors has shown that:

  • PRP is highly variable between individual donors (patients)
  • Preparation methods impact the composition of PRP
  • Donors can have high levels of microparticles, microaggregates and immobilized platelets
  • Platelet count is highly variable within a normal range

Practitioners using PRP can employ ThromboLUX to better understand their production of PRP, implement quality control, and to gain insight about the PRP when evaluating the effectiveness of a treatment.

When can ThromboLUX be used?

ThromboLUX can be used at any stage of PRP production. The test requires a single drop (100 μL) of sample to perform the quick seven minute analysis. 

Figure 5.9 Samples for PRP testing

Figure 5.9 Samples for PRP testing